MOLECULAR PRINTING SYSTEM

MODEL NANO ENABLER

The Nano eNabler™ system is a highly flexible molecular printer that can dispense minute volumes of liquid at defined positions to create patterns of spots or lines with high spatial accuracy. The system is based on BioForce’s proprietary FEMTO (Fluidics Enhanced Molecular Transfer Operation) process. This technology enables the deposition of the 1-30 um sample droplets quickly, precisely and with reduced clogging.

The core of the Nano eNabler system is a compact precision motion control platform mated to a microfabricated surface patterning tool (SPT™) with integrated microfluidics. Molecules to be printed are loaded into the SPT™ reservoirs by pipet for the ultimate in simplicity. A variable intensity laser and position-sensitive photodetector are employed to monitor the contact time and force between the SPT™ cantilever and the surface being patterned. In this way, the user is able to precisely control the volume of liquid being dispensed and the resulting feature size. Sophisticated electronics and a powerful user interface provide millisecond control over each deposition event. To ensure consistent printing, a constant humidity is maintained by an environmental chamber and computer-controlled humidifier. The entire process can be viewed through a high resolution optical zoom microscope and CCD camera with video displayed within the NanoWare™ software. To minimize the instrument’s footprint, the computer has been integrated into the controller.

System Features

Applications

  • Spot size: 1-50 microns (attoliter to femtoliter)
  • XY Travel: 50 mm
  • XY Resolution: 20 nm
  • Printing cycle: 100ms
  • Built-in optical microscope
  • Control software
  • Multiplexing ability

Benefits

  • Larger patterns: 250,000 times more XY patterning area than Scanning Probe Lithography systems
  • Faster printing: Direct molecular deposition via FEMTO does not rely on diffusion
  • Less frustration: Open channel SPT(TM) print cartridges exhibit less clogging than ink-jets or nano-pipettes
  • More capabilities: Superior alignment and multiplexing compared to microcontact printing
  • Greater flexibility: Make design changes in software without new photomasks or PDMS molds

Cell Patterning
By patterning substrates with sub cellular domains of proteins, it is possible to influence individual cells in a variety of ways. Signaling proteins can be precisely placed and cells grown on the surface contacting deposited materials. Patterning cellular adhesion molecules and blocking the surrounding area make it possible to create arrays of individual or small groups of cells. The highly flexible nature of the instruments allows immediate changes to the patterning of a surface.

Biosensor Functionalization
The Nano eNablerTM system can be used to functionalize transducing domains in a large variety of biosensors. Small spot sizes mean more testing domains on smaller tests.

Small Volume Biomolecular Assays
Biomarker detection and quantification are a good example of small-volume assays with applications including cancer detection, staging, and monitoring, as well as toxicity detection.

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